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KMID : 0829220170410020055
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Korean Journal of Oral and Maxillofacial Pathology
2017 Volume.41 No. 2 p.55 ~ p.62
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miR-4708 Enhances Paclitaxel Sensitivity through Modulating Expression of Multidrug Resistance Protein-1
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Choi Jang-Yeol
Ryu Ji-Won
Ahn Sang-Gun
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Abstract
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Multidrug resistance (MDR) remains one of the most significant obstacles in various cancer treatment, and this process often involves dysregulation of the number of micro-RNAs. The aim of this study was to explore the role of miR-4708 on the regulation of MDR-1 expression and the regulation of multidrug resistance (MDR) to chemotherapeutic drugs. Luciferase reporter assays demonstrated that miR-4708 directly binds MDR-1 3’-UTR and down-regulated reporter luciferase activity. The mRNA and protein expression levels of MDR1 were significantly decreased following miR-4708 overexpression. Additionally, the accumulation of rhodamine-123 in paclitacel resistant FaDu cells following miR-4708 transfection was significantly increased compared with control, indicating that the efflux capacity was reduced. These results demonstrated that miR-4708 could be involved in the regulation of MDR via targeting MDR-1 and may provide a potential strategy for reversing drug resistance in oral cancer.
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KEYWORD
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miR-4708, FaDu, MDR1, Paclitaxel
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